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Today's question of the day:
Which cell will show the slowest fluorescence recovery after photobleaching?
Fluorescence recovery after photobleaching (FRAP) measures the rate at which fluorescent molecules move into a bleached area within a cell. E-cadherin is a cell adhesion molecule that forms adherens junctions between adjacent cells. These junctions anchor cells together and play a role in cell-cell adhesion and signaling. Hence, it is less mobile and its movement within the cell membrane is likely slower, leading to a slower fluorescence recovery after photobleaching.