The technique of co-immunofluorescence will indicate if two proteins are in the same area of a tissue or cell. How does it work?

Co-immunofluorescence is a technique that uses two primary antibodies specific for different proteins, each detected by a secondary antibody conjugated to a distinct fluorophore (color). The sample is visualized under a fluorescence microscope, and co-localization is determined if both fluorescent signals overlap, indicating that the two proteins are present in the same area. A is incorrect because two antibodies with the same color cannot distinguish the proteins. B is incorrect because a single antibody cannot recognize two different proteins. C is incorrect because protein co-localization cannot be determined based solely on inherent color without antibodies